Rheumatology in practice

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Reflections on anti-tumour necrosis factor therapy
RDIP, Volume 6, Number 2.

Many years ago, I did an intercalated degree in clinical immunology and oncology. My own particular project was to identify natural killer (NK) cells in the rat. I undertook this at the Paterson laboratories at the Christie Hospital in Manchester. At that time (1982), NK cells were identifiable under the microscope, but were difficult to harvest. I used a technique of differential centrifugation across a Percoll gradient to separate NK cells from other lymphocytes. They could then be used for other research projects.

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